Making a Slide

So. Here's how I make a slide.


1. Line up embryos with a probe (pointy thing).

2. Put them on a slide (see the little white smudge in the middle?).

We put them in halocarbon oil so they can still breathe!

3. Put the slide in the microscope.

4. Turn on the laser. (488nm = blue)

5. Viola! (10x lens)

This is basically what I do for my experiment. Today, I did a time lapse series. I took pictures every hour to see how GFP expression varies over time. Hopefully, I'll be able to fit a line or a curve to my data points tomorrow.